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验证数据展示

  • WB analysis using 83463-8-RR

    WB analysis using 83463-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83463-8-RR

    IHC staining of mouse liver using 83463-8-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-251 using 83463-8-RR

    FC experiment of U-251 using 83463-8-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83463-8-RR

    Affinity and Kinetic Characterization of 83463-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

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TRMT2A Recombinant monoclonal antibody

TRMT2A Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83463-8-RR

产品说明书

CloneNo. 240444C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, A431 cells, MCF-7 cells, MOLT-4 cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83463-8-RR targets TRMT2A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9112

Product name: Recombinant human TRMT2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 277-625 aa of BC013352

Sequence: DTVHIPEATKQVVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWAQLDAGSMVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLASQHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERVEHPNGTGVLGPHSPPAQPTPGPPDNTLQETGTFPSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
别名 tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-
计算分子量 625 aa, 69 kDa
观测分子量 42-44 kDa, 68-75 kDa
GenBank蛋白编号BC013352
基因名称 TRMT2A
Gene ID (NCBI) 27037
RRIDAB_3671094
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IZ69
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

实验方案

Product Specific Protocols
WB protocol for TRMT2A antibody 83463-8-RRDownload protocol
IHC protocol for TRMT2A antibody 83463-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols