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验证数据展示

  • WB analysis using 83463-8-RR (same clone as 83463-8-PBS)

    WB analysis using 83463-8-RR (same clone as 83463-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83463-8-RR (same clone as 83463-8-PBS)

    IHC staining of mouse liver using 83463-8-RR (same clone as 83463-8-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83463-8-RR (same clone as 83463-8-PBS)

    FC experiment of U-251 using 83463-8-RR (same clone as 83463-8-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83463-8-RR

    Affinity and Kinetic Characterization of 83463-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

TRMT2A Recombinant monoclonal antibody, PBS Only

TRMT2A Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83463-8-PBS

产品说明书

CloneNo. 240444C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

240444C2, EC:2.1.1.-, EC:2.1.1.35, HTF9C, mRNA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83463-8-PBS targets TRMT2A in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9112

Product name: Recombinant human TRMT2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 277-625 aa of BC013352

Sequence: DTVHIPEATKQVVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWAQLDAGSMVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLASQHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERVEHPNGTGVLGPHSPPAQPTPGPPDNTLQETGTFPSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
别名 240444C2, EC:2.1.1.-, EC:2.1.1.35, HTF9C, mRNA
计算分子量 625 aa, 69 kDa
观测分子量 42-44 kDa, 68-75 kDa
GenBank蛋白编号BC013352
基因名称 TRMT2A
Gene ID (NCBI) 27037
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IZ69
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

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