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CoraLite® Plus 488-conjugated TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5D8C7

Cat No : CL488-60205

Print datasheet

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

验证数据展示

  • FC experiment of HeLa using CL488-60205

    FC experiment of HeLa using CL488-60205

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL488-60205

    FC experiment of HepG2 using CL488-60205

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60205-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60205 targets TRIP6 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen TRIP6 fusion protein Ag15149 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name thyroid hormone receptor interactor 6
Synonyms OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1
Calculated Molecular Weight 476 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC004249
Gene Symbol TRIP6
Gene ID (NCBI) 7205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15654
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TRIP6 antibody CL488-60205Download protocol
Standard Protocols
Click here to view our Standard Protocols