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验证数据展示

  • FC experiment of HeLa using CL488-60205

    FC experiment of HeLa using CL488-60205

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL488-60205

    FC experiment of HepG2 using CL488-60205

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trip6 Monoclonal Antibody (CL488-60205, Clone:5D8C7)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for FC (Intra)
Cat No. CL488-60205

产品说明书

CloneNo. 5D8C7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

FC (Intra)

别名

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60205-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60205 targets TRIP6 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15149

Product name: Recombinant human TRIP6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 127-476 aa of BC004249

Sequence: PPPAYRTGSLKPNPASPLPASPYGGPTPASYTTASTPAGPAFPVQVKVAQPVRGCGPPRRGASQASGPLPGPHFPLPGRGEVWGPGYRSQREPGPGAKEEAAGVSGPAGRGRGGEHGPQVPLSQPPEDELDRLTKKLVHDMNHPPSGEYFGQCGGCGEDVVGDGAGVVAFDRVFHVGCFVCSTCRAQLRGQHFYAVERRAYCEGCYVATLEKCATCSQPILDRILRAMGKAYHPGCFTCVVCHRGLDGIPFTVDATSQIHCIEDFHRKFAPRCSVCGGAIMPEPGQEETVRIVALDRSFHIGCYKCEECGLLLSSEGECQGCYPLDGHILCKACSAWRIQELSATVTTDC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thyroid hormone receptor interactor 6
别名 OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1
计算分子量 476 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC004249
基因名称 TRIP6
Gene ID (NCBI) 7205
RRIDAB_3672861
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15654
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TRIP6 antibody CL488-60205Download protocol
Standard Protocols
Click here to view our Standard Protocols
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