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验证数据展示

  • WB analysis using 29907-1-AP (same clone as 29907-1-PBS)

    WB analysis using 29907-1-AP (same clone as 29907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29907-1-AP (TRIM69 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29907-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29907-1-AP (same clone as 29907-1-PBS)

    IHC staining of mouse cerebellum using 29907-1-AP (same clone as 29907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29907-1-AP (TRIM69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29907-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29907-1-AP (same clone as 29907-1-PBS)

    IHC staining of mouse cerebellum using 29907-1-AP (same clone as 29907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29907-1-AP (TRIM69 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29907-1-PBS in a different storage buffer formulation.

TRIM69 Polyclonal antibody, PBS Only

TRIM69 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29907-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29907-1-PBS targets TRIM69 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31775

Product name: Recombinant human TRIM69 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC033314

Sequence: MEEELAIQQGQLETTLKELQTLRNMQKEAIAAHKENKLHLQQHVSMEFLKLHQFLHSKEKDILTELREEGKALNEEMELNLSQLQEQCLLAKDMLVSIQAKTEQQNSFDFLKDITTLLHSLEQGMKVLATRELISRKLNLGQYKGPIQYMVWREMQDTLCPGLSPLTLDPKTAHPNLVLSKSQTSVWHGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 69
别名
计算分子量 500 aa, 57 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC033314
基因名称 TRIM69
Gene ID (NCBI) 140691
RRIDAB_2935487
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WT6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

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