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验证数据展示

  • WB analysis using 29907-1-AP

    WB analysis using 29907-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29907-1-AP (TRIM69 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 29907-1-AP

    IHC staining of mouse cerebellum using 29907-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29907-1-AP (TRIM69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29907-1-AP

    IHC staining of mouse cerebellum using 29907-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29907-1-AP (TRIM69 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRIM69 Polyclonal antibody

TRIM69 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29907-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, SH-SY5Y cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29907-1-AP targets TRIM69 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 TRIM69 fusion protein Ag31775 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 69
别名 HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69
计算分子量 500 aa, 57 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC033314
基因名称 TRIM69
Gene ID (NCBI) 140691
RRIDAB_2935487
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for TRIM69 antibody 29907-1-APDownload protocol
IHC protocol for TRIM69 antibody 29907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols