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验证数据展示

  • WB analysis using 86728-1-RR

    WB analysis using 86728-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of OVCAR-3 using 86728-1-RR

    WB analysis of OVCAR-3 using 86728-1-RR

    OVCAR-3 cells were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86728-1-RR

    WB analysis using 86728-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86728-1-RR

    Affinity and Kinetic Characterization of 86728-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86728-1-RR against Human TRIM47 were performed. The affinity constant is 1.77 nM.

TRIM47 Recombinant monoclonal antibody

TRIM47 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86728-1-RR

产品说明书

CloneNo. 251436A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse colon tissue, OVCAR-3 cells, U2OS cells, SMMC-7721 cells, PC-3 cells, rat colon tissue
Planning an IHC experiment? We recommend our IHCeasy TRIM47 Ready-To-Use IHC Kit. TRIM47 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86728-1-RR targets TRIM47 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22868

Product name: Recombinant human TRIM47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC017299

Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 47
别名 E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47
计算分子量 638 aa, 70 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC017299
基因名称 TRIM47
Gene ID (NCBI) 91107
RRIDAB_3745084
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96LD4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381).

实验方案

Product Specific Protocols
WB protocol for TRIM47 antibody 86728-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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