验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, U2OS cells, mouse colon tissue, rat colon tissue |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, mouse colon tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26885-1-AP targets TRIM47 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22868 Product name: Recombinant human TRIM47 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017299 Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 47 |
| 别名 | E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47 |
| 计算分子量 | 638 aa, 70 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC017299 |
| 基因名称 | TRIM47 |
| Gene ID (NCBI) | 91107 |
| RRID | AB_2880669 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LD4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| IP protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| WB protocol for TRIM47 antibody 26885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.
| ||
Acta Pharm Sin B GRK2 activates TRAF2-NF-κB signalling to promote hyperproliferation of fibroblast-like synoviocytes in rheumatoid arthritis | ||
Cell Death Discov Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma | ||
Acta Pharmacol Sin Trim47 protects against hypoxic-ischaemic brain injury in neonatal rats by reducing brain microvascular endothelial inflammation and BBB disruption by interacting with ZO1.
| ||
Free Radic Biol Med TRIM47-CDO1 axis dictates hepatocellular carcinoma progression by modulating ferroptotic cell death through the ubiquitin‒proteasome system | ||
Cell Mol Life Sci TRIM47 drives metabolic reprogramming and tumor progression in Nasopharyngeal Carcinoma via K48-linked ubiquitination and degradation of SDHB. |
