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验证数据展示

  • IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TRIM36 antibody (25913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    IHC staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of rat testis using 25913-1-AP (same clone as 25913-1-PBS)

    IHC staining of rat testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    IF Staining of mouse testis using 25913-1-AP (same clone as 25913-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TRIM36 antibody (25913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25913-1-PBS in a different storage buffer formulation.

TRIM36 Polyclonal antibody, PBS Only

TRIM36 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 25913-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, Indirect ELISA

别名

E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM36 Ready-To-Use IHC Kit. TRIM36 primary antibody included.

产品信息

25913-1-PBS targets TRIM36 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23240

Product name: Recombinant human TRIM36 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-215 aa of BC130334

Sequence: ESTKSCMDCSASYCNECFKIHHPWGTIKAQHEYVGPTTNFRPKILMCPEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 36
别名 E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36
观测分子量83 kDa
GenBank蛋白编号BC130334
基因名称 TRIM36
Gene ID (NCBI) 55521
RRIDAB_2880293
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ86
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM36, a member of the tripartite motif (TRIM) family of RING-containing proteins, also known as Haprin, is a microtubule-associated E3 ubiquitin ligase that plays a role in cytoskeletal organization, which was first discovered for its abundance in testis and found to be implicated in the spermatozoa acrosome reaction (PMID: 35053362, 30944633). TRIM36 was reported as a novel androgen signaling target gene and is upregulated in prostate cancer (PMID: 29449534).

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