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验证数据展示

  • WB analysis using 25913-1-AP

    WB analysis using 25913-1-AP

    mouse testis tissue was subjected to SDS PAGE followed by western blot with 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25913-1-AP

    IHC staining of mouse testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of mouse testis using 25913-1-AP

    IHC staining of mouse testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of rat testis using 25913-1-AP

    IHC staining of rat testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included

TRIM36 Polyclonal antibody

TRIM36 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25913-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRIM36 Ready-To-Use IHC Kit. TRIM36 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25913-1-AP targets TRIM36 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23240

Product name: Recombinant human TRIM36 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-215 aa of BC130334

Sequence: ESTKSCMDCSASYCNECFKIHHPWGTIKAQHEYVGPTTNFRPKILMCPEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 36
别名 E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36
观测分子量83 kDa
GenBank蛋白编号BC130334
基因名称 TRIM36
Gene ID (NCBI) 55521
RRIDAB_2880293
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM36, a member of the tripartite motif (TRIM) family of RING-containing proteins, also known as Haprin, is a microtubule-associated E3 ubiquitin ligase that plays a role in cytoskeletal organization, which was first discovered for its abundance in testis and found to be implicated in the spermatozoa acrosome reaction (PMID: 35053362, 30944633). TRIM36 was reported as a novel androgen signaling target gene and is upregulated in prostate cancer (PMID: 29449534).

实验方案

Product Specific Protocols
WB protocol for TRIM36 antibody 25913-1-APDownload protocol
IHC protocol for TRIM36 antibody 25913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols