• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 25913-1-AP

    WB analysis using 25913-1-AP

    mouse testis tissue was subjected to SDS PAGE followed by western blot with 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25913-1-AP

    IHC staining of mouse testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of mouse testis using 25913-1-AP

    IHC staining of mouse testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included
  • IHC staining of rat testis using 25913-1-AP

    IHC staining of rat testis using 25913-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25913-1-AP (TRIM36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM36 antibody included

TRIM36 Polyclonal antibody

TRIM36 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25913-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRIM36 Ready-To-Use IHC Kit. TRIM36 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25913-1-AP targets TRIM36 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRIM36 fusion protein Ag23240 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 36
别名 E3 ubiquitin-protein ligase TRIM36, EC:2.3.2.27, RING-type E3 ubiquitin transferase TRIM36, RNF98, tripartite motif containing 36
观测分子量83 kDa
GenBank蛋白编号BC130334
基因名称 TRIM36
Gene ID (NCBI) 55521
RRIDAB_2880293
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM36, a member of the tripartite motif (TRIM) family of RING-containing proteins, also known as Haprin, is a microtubule-associated E3 ubiquitin ligase that plays a role in cytoskeletal organization, which was first discovered for its abundance in testis and found to be implicated in the spermatozoa acrosome reaction (PMID: 35053362, 30944633). TRIM36 was reported as a novel androgen signaling target gene and is upregulated in prostate cancer (PMID: 29449534).

实验方案

Product Specific Protocols
WB protocol for TRIM36 antibody 25913-1-APDownload protocol
IHC protocol for TRIM36 antibody 25913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols