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验证数据展示

  • WB analysis using 85364-3-RR (same clone as 85364-3-PBS)

    WB analysis using 85364-3-RR (same clone as 85364-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85364-3-RR (same clone as 85364-3-PBS)

    IF Staining of HepG2 using 85364-3-RR (same clone as 85364-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 85364-3-RR (same clone as 85364-3-PBS)

    WB analysis of COLO 320 using 85364-3-RR (same clone as 85364-3-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85364-3-PBS in a different storage buffer formulation.

TRIM33 Recombinant antibody, PBS Only

TRIM33 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85364-3-PBS

产品说明书

CloneNo. 242728H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Protein Rfg7, KIAA1113, Ectodermin homolog, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM33

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85364-3-PBS targets TRIM33 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 33
别名 Protein Rfg7, KIAA1113, Ectodermin homolog, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM33
计算分子量 122 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_015906
基因名称 TRIM33
Gene ID (NCBI) 51592
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPN9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).