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验证数据展示

  • WB analysis using 85364-3-RR

    WB analysis using 85364-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 85364-3-RR

    WB analysis of COLO 320 using 85364-3-RR

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 85364-3-RR

    IF Staining of A431 using 85364-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TRIM33 Recombinant monoclonal antibody

TRIM33 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85364-3-RR

产品说明书

CloneNo. 242728H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242728H2, E3 ubiquitin-protein ligase TRIM33, EC:2.3.2.27, Ectodermin homolog, KIAA1113

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, COLO 320 cells, MOLT-4 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85364-3-RR targets TRIM33 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 33
别名 242728H2, E3 ubiquitin-protein ligase TRIM33, EC:2.3.2.27, Ectodermin homolog, KIAA1113
计算分子量 122 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_015906
基因名称 TRIM33
Gene ID (NCBI) 51592
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).

实验方案

Product Specific Protocols
IF protocol for TRIM33 antibody 85364-3-RRDownload protocol
WB protocol for TRIM33 antibody 85364-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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