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验证数据展示

  • IF Staining of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    IF Staining of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    IF Staining of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    FC experiment of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    FC experiment of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84927-6-RR

    Affinity and Kinetic Characterization of 84927-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84927-6-RR against Human TRIM27 were performed. The affinity constant is 0.653 nM.

TRIM27 Recombinant monoclonal antibody, PBS Only

TRIM27 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84927-6-PBS

产品说明书

CloneNo. 242538H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

242538H5, EC:2.3.2.27, RFP, RNF76, TRIM

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM27 Ready-To-Use IHC Kit. TRIM27 primary antibody included.

产品信息

84927-6-PBS targets TRIM27 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2849

Product name: Recombinant human TRIM27 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 165-513 aa of BC013580

Sequence: EQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 27
别名 242538H5, EC:2.3.2.27, RFP, RNF76, TRIM
计算分子量 513 aa, 58 kDa
GenBank蛋白编号BC013580
基因名称 TRIM27
Gene ID (NCBI) 5987
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP14373
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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