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验证数据展示

  • IF Staining of HepG2 using CL488-67136

    IF Staining of HepG2 using CL488-67136

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TRIM21 antibody (CL488-67136, Clone: 1E8B9 ) at dilution of 1:100. Blue (DAPI).

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  • KD/KO验证

CoraLite® Plus 488-conjugated TRIM21 Monoclonal antibody

TRIM21 Monoclonal Antibody for IF/ICC
Cat No. CL488-67136

产品说明书

CloneNo. 1E8B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM21 Ready-To-Use IHC Kit. TRIM21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67136-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67136 targets TRIM21 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 TRIM21 fusion protein Ag28377 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 21
别名 52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21
计算分子量 475 aa, 54 kDa
观测分子量 52 kDa
GenBank蛋白编号BC010861
基因名称 TRIM21
Gene ID (NCBI) 6737
RRIDAB_2919426
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP19474
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRIM21 antibody CL488-67136Download protocol
Standard Protocols
Click here to view our Standard Protocols