• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TRIM2 Monoclonal antibody

TRIM2 Monoclonal Antibody for IF-P, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

IF-P, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1H9C2

Cat No : 67342-1-Ig

Print datasheet

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

验证数据展示

  • IF Staining of mouse brain using 67342-1-Ig

    IF Staining of mouse brain using 67342-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67342-1-Ig

    IF Staining of mouse brain using 67342-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of rat brain using 67342-1-Ig

    WB analysis of rat brain using 67342-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67342-1-Ig

    IHC staining of mouse brain using 67342-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67342-1-Ig

    IHC staining of mouse brain using 67342-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, mouse cerebellum tissue, pig brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67342-1-Ig targets TRIM2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF-P, IHC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman
Immunogen TRIM2 fusion protein Ag14637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 2
Synonyms KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2
Calculated Molecular Weight 744 aa, 82 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC011052
Gene Symbol TRIM2
Gene ID (NCBI) 23321
RRIDAB_2882600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9C040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

实验方案

Product Specific Protocols
WB protocol for TRIM2 antibody 67342-1-IgDownload protocol
IHC protocol for TRIM2 antibody 67342-1-IgDownload protocol
IF protocol for TRIM2 antibody 67342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Cell Int

TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.

Authors - Zhaoxian Lin
View Article