×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87377-3-RR

    WB analysis using 87377-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87377-3-RR (TRIM16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87377-3-RR

    IF Staining of HeLa using 87377-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A549 using 87377-3-RR

    IF Staining of A549 using 87377-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TRIM16 Recombinant monoclonal antibody

TRIM16 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87377-3-RR

产品说明书

CloneNo. 252519D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HaCaT cells
Positive IF/ICC detected inHeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM16 Ready-To-Use IHC Kit. TRIM16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87377-3-RR targets TRIM16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21406

Product name: Recombinant human TRIM16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC015674

Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 16
别名 E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16
计算分子量 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC015674
基因名称 TRIM16
Gene ID (NCBI) 10626
RRIDAB_3745496
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95361
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902). Ramos is the negative control.

实验方案

Product Specific Protocols
IF protocol for TRIM16 antibody 87377-3-RRDownload protocol
WB protocol for TRIM16 antibody 87377-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...