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验证数据展示

  • WB analysis using 15742-1-AP (same clone as 15742-1-PBS)

    WB analysis using 15742-1-AP (same clone as 15742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • WB analysis of L02 using 15742-1-AP (same clone as 15742-1-PBS)

    WB analysis of L02 using 15742-1-AP (same clone as 15742-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15742-1-AP (same clone as 15742-1-PBS)

    IF Staining of HeLa using 15742-1-AP (same clone as 15742-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM14 antibody (15742-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM14 Polyclonal antibody, PBS Only

TRIM14 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15742-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15742-1-PBS targets TRIM14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8158

Product name: Recombinant human TRIM14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-346 aa of BC006333

Sequence: MAGAATGSRTPGRSELVEGCGWRCPEHGDRVAELFCRRCRRCVCALCPVLGAHRGHPVGLALEAAVHVQKLSQECLKQLAIKKQQHIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDKNTQLTLQVYREQADSCREQLDIMNDLSNRVWSISQEPDPVQRLQAYTATEQEMQQQMSLGELCHPVPLSFEPVKSFFKGLVEAVESTLQTPLDIRLKESINCQLSDPSSTKPGTLLKTSPSPERSLLLKYARTPTLDPDTMHARLRLSADRLTVRCGLLGSLGPVPVLRFDALWQVLARDCFATGRHYWEVDVQEAGAGWWVGAAYASLRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tripartite motif-containing 14
别名
计算分子量 442 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC006333
基因名称 TRIM14
Gene ID (NCBI) 9830
RRIDAB_2209324
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14142
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

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