验证数据展示
经过测试的应用
Positive WB detected in | L02 cells, HL-60 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15742-1-AP targets TRIM14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | TRIM14 fusion protein Ag8158 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tripartite motif-containing 14 |
别名 | Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129 |
计算分子量 | 442 aa, 50 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC006333 |
基因名称 | TRIM14 |
Gene ID (NCBI) | 9830 |
RRID | AB_2209324 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14142 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.
实验方案
Product Specific Protocols | |
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WB protocol for TRIM14 antibody 15742-1-AP | Download protocol |
IHC protocol for TRIM14 antibody 15742-1-AP | Download protocol |
IF protocol for TRIM14 antibody 15742-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells
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Sci Rep TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.
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Sci Rep TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.
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Sci Rep Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation. | ||
Carcinogenesis TRIM14 suppressed the progression of NSCLC via hexosamine biosynthesis pathway | ||
Front Pharmacol Hypoxia-Induced Upregulation of lncRNA ELFN1-AS1 Promotes Colon Cancer Growth and Metastasis Through Targeting TRIM14 via Sponging miR-191-5p. |