验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13300-1-AP targets TRIB3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, monkey |
| 免疫原 |
CatNo: Ag4085 Product name: Recombinant human TRIB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC027484 Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tribbles homolog 3 (Drosophila) |
| 别名 | SKIP3, SINK, p65-interacting inhibitor of NF-kappa-B, NIPK, Neuronal cell death-inducible putative kinase |
| 计算分子量 | 358 aa, 40 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC027484 |
| 基因名称 | TRIB3 |
| Gene ID (NCBI) | 57761 |
| RRID | AB_10641988 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RU7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| WB protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Mol Hepatol MET promotes hepatocellular carcinoma development through the promotion of TRIB3-mediated FOXO1 degradation
| ||
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
| ||
Autophagy ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis | ||
Cell Death Dis TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis | ||
Cell Death Dis RNA N6-methyladenosine demethylase FTO promotes diabetic wound healing through TRIB3-mediated autophagy in an m6A-YTHDF2-dependent manner |
