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验证数据展示

  • WB analysis using 25332-1-AP (same clone as 25332-1-PBS)

    WB analysis using 25332-1-AP (same clone as 25332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25332-1-AP (TRH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25332-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 25332-1-AP (same clone as 25332-1-PBS)

    IHC staining of human brain using 25332-1-AP (same clone as 25332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25332-1-AP (TRH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25332-1-PBS in a different storage buffer formulation.

TRH Polyclonal antibody, PBS Only

TRH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25332-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Prothyroliberin, Pro-thyrotropin-releasing hormone, Protirelin, Thyrotropin-releasing hormone, TRF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25332-1-PBS targets TRH in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12623

Product name: Recombinant human TRH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-242 aa of BC074889

Sequence: MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thyrotropin-releasing hormone
别名 Prothyroliberin, Pro-thyrotropin-releasing hormone, Protirelin, Thyrotropin-releasing hormone, TRF
计算分子量 242 aa, 27 kDa
观测分子量27-33 kDa
GenBank蛋白编号BC074889
基因名称 TRH
Gene ID (NCBI) 7200
RRIDAB_3085786
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20396
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).

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