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验证数据展示

  • WB analysis using 25332-1-AP

    WB analysis using 25332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25332-1-AP (TRH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 25332-1-AP

    IF Staining of mouse cerebellum using 25332-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using TRH antibody (25332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

TRH Polyclonal antibody

TRH Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 25332-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25332-1-AP targets TRH in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 TRH fusion protein Ag12623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thyrotropin-releasing hormone
别名 Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor
计算分子量 242 aa, 27 kDa
观测分子量27-33 kDa
GenBank蛋白编号BC074889
基因名称 TRH
Gene ID (NCBI) 7200
RRIDAB_3085786
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20396
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).

实验方案

Product Specific Protocols
WB protocol for TRH antibody 25332-1-APDownload protocol
IF protocol for TRH antibody 25332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols