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验证数据展示

  • FC experiment of human peripheral blood leukocytes using Biotin-FcA98154

    FC experiment of human peripheral blood leukocytes using Biotin-FcA98154

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human TREM-1/CD354 Rabbit RecAb (Biotin-FcA98154, Clone:241279D10) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with FcZero-rAb® CoraLite® Plus 405 Anti-Human CD16 (3G8) Rabbit Recombinant Antibody (CL405-FcA65612, Clone: 3G8). Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using Biotin-FcA98154

    FC experiment of human peripheral blood leukocytes using Biotin-FcA98154

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human TREM-1/CD354 Rabbit RecAb (Biotin-FcA98154, Clone: 241279D10) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® Biotin Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody

TREM-1/CD354 FcZero-rAb® Recombinant Antibody for FC, ELISA
Cat No. Biotin-FcA98154

产品说明书

CloneNo. 241279D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, ELISA

别名

TREM1, CD354, TREM 1

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-FcA98154 targets TREM-1/CD354 in FC, ELISA applications and shows reactivity with human samples.

经测试应用 FC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1438

Product name: Recombinant Human TREM-1/CD354 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-200 aa of NM_018643.5

Sequence: ATKLTEEKYELKEGQTLDVKCDYTLEKFASSQKAWQIIRDGEMPKTLACTERPSKNSHPVQVGRIILEDYHDHGLLRVRMVNLQVEDSGLYQCVIYQPPKEPHMLFDRIRLVVTKGFSGTPGSNENSTQNVYKIPPTTTKALCPLYTSPRTVTQAPPKSTADVSTPDSEINLTNVTDIIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 triggering receptor expressed on myeloid cells 1
别名 TREM1, CD354, TREM 1
计算分子量26kDa
GenBank蛋白编号NM_018643.5
基因名称 TREM1
Gene ID (NCBI) 54210
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NP99-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.

实验方案

Product Specific Protocols
FC protocol for Biotin TREM-1/CD354 antibody Biotin-FcA98154Download protocol
Standard Protocols
Click here to view our Standard Protocols
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