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验证数据展示

  • WB analysis using 27549-1-AP (same clone as 27549-1-PBS)

    WB analysis using 27549-1-AP (same clone as 27549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

    IP experiment of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

    IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

    IHC staining of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 27549-1-AP (same clone as 27549-1-PBS)

    IHC staining of rat brain using 27549-1-AP (same clone as 27549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

TRAPPC10 Polyclonal antibody, PBS Only

TRAPPC10 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27549-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27549-1-PBS targets TRAPPC10 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26900

Product name: Recombinant human TRAPPC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-303 aa of BC101728

Sequence: IVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSDRCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWSFCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNFGAGDGANWLTFFCQPVKSWNGLILRKPIDMEKRESIQRREATL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein particle complex 10
别名
计算分子量 1259 aa, 142 kDa
观测分子量 142 kDa
GenBank蛋白编号BC101728
基因名称 TRAPPC10
Gene ID (NCBI) 7109
RRIDAB_3085968
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48553
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.

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