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验证数据展示

  • WB analysis using 27549-1-AP

    WB analysis using 27549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27549-1-AP

    IP experiment of mouse brain using 27549-1-AP

    IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug.

  • IHC staining of mouse brain using 27549-1-AP

    IHC staining of mouse brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 27549-1-AP

    IHC staining of rat brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAPPC10 Polyclonal antibody

TRAPPC10 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27549-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

EHOC 1, EHOC1, GT334, Protein GT334, TMEM1, TRAPP subunit TMEM1, TRAPPC10, TRS130, TRS30

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27549-1-AP targets TRAPPC10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26900

Product name: Recombinant human TRAPPC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-303 aa of BC101728

Sequence: IVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSDRCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWSFCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNFGAGDGANWLTFFCQPVKSWNGLILRKPIDMEKRESIQRREATL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein particle complex 10
别名 EHOC 1, EHOC1, GT334, Protein GT334, TMEM1, TRAPP subunit TMEM1, TRAPPC10, TRS130, TRS30
计算分子量 1259 aa, 142 kDa
观测分子量 142 kDa
GenBank蛋白编号BC101728
基因名称 TRAPPC10
Gene ID (NCBI) 7109
RRIDAB_3085968
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48553
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.

实验方案

Product Specific Protocols
IHC protocol for TRAPPC10 antibody 27549-1-APDownload protocol
IP protocol for TRAPPC10 antibody 27549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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