• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TRAPPC10 Polyclonal antibody

TRAPPC10 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat No : 27549-1-AP

Print datasheet

Synonyms

EHOC 1, EHOC1, GT334, Protein GT334, TMEM1, TRAPP subunit TMEM1, TRAPPC10, TRS130, TRS30

验证数据展示

  • WB analysis using 27549-1-AP

    WB analysis using 27549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27549-1-AP

    IP experiment of mouse brain using 27549-1-AP

    IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug.

  • IHC staining of mouse brain using 27549-1-AP

    IHC staining of mouse brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 27549-1-AP

    IHC staining of rat brain using 27549-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27549-1-AP targets TRAPPC10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, ELISA
Tested Reactivity human, mouse, rat
Immunogen TRAPPC10 fusion protein Ag26900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trafficking protein particle complex 10
Synonyms EHOC 1, EHOC1, GT334, Protein GT334, TMEM1, TRAPP subunit TMEM1, TRAPPC10, TRS130, TRS30
Calculated Molecular Weight 1259 aa, 142 kDa
Observed Molecular Weight 142 kDa
GenBank Accession NumberBC101728
Gene Symbol TRAPPC10
Gene ID (NCBI) 7109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.

实验方案

Product Specific Protocols
IHC protocol for TRAPPC10 antibody 27549-1-APDownload protocol
IP protocol for TRAPPC10 antibody 27549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols