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验证数据展示

  • WB analysis using 10278-1-AP (same clone as 10278-1-PBS)

    WB analysis using 10278-1-AP (same clone as 10278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

    IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

    IHC staining of human breast cancer using 10278-1-AP (same clone as 10278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10278-1-AP (same clone as 10278-1-PBS)

    IF Staining of HepG2 using 10278-1-AP (same clone as 10278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRAPB/SSR2 antibody (10278-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10278-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRAPB/SSR2 Polyclonal antibody, PBS Only

TRAPB/SSR2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10278-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10278-1-PBS targets TRAPB/SSR2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0304

Product name: Recombinant human TRAPB, SSR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-175 aa of BC000341

Sequence: MRLLSFVVLALFAVTQAEEGARLLASKSLLNRYAVEGRDLTLQYNIYNVGSSAALDVELSDDSFPPEDFGIVSGMLNVKWDRIAPASNVSHTVVLRPLKAGYFNFTSATITYLAQEDGPVVIGSTSAPGQGGILAQREFDRRFSPHFLDWAAFGVMTLPSIGIPLLLWYSSKRKY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal sequence receptor, beta (translocon-associated protein beta)
别名
计算分子量 20 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC000341
基因名称 TRAPB
Gene ID (NCBI) 6746
RRIDAB_2195624
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43308
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

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