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TRAPB/SSR2 Polyclonal antibody

TRAPB/SSR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10278-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

SSR2, Translocon-associated protein subunit beta, TLAP, SSR-beta, SSR beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10278-1-AP targets TRAPB/SSR2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 TRAPB/SSR2 fusion protein Ag0304 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal sequence receptor, beta (translocon-associated protein beta)
别名 SSR2, Translocon-associated protein subunit beta, TLAP, SSR-beta, SSR beta
计算分子量 20 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC000341
基因名称 TRAPB
Gene ID (NCBI) 6746
RRIDAB_2195624
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43308
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

实验方案

Product Specific Protocols
WB protocol for TRAPB/SSR2 antibody 10278-1-APDownload protocol
IHC protocol for TRAPB/SSR2 antibody 10278-1-APDownload protocol
IF protocol for TRAPB/SSR2 antibody 10278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.

Authors - Xianyan Hu
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
humanWB

Diabetes

The Role of TRAPγ/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum.

Authors - Xiaoxi Xu
ratWB, IP

FASEB J

Deficient endoplasmic reticulum translocon-associated protein complex limits the biosynthesis of proinsulin and insulin.

Authors - Yumeng Huang
  • KD Validated
humanWB,IHC

J Cell Mol Med

Overexpression of SSR2 promotes proliferation of liver cancer cells and predicts prognosis of patients with hepatocellular carcinoma.

Authors - Fengsui Chen
  • KD Validated
humanIHC

J Immunol Res

Endoplasmic Reticulum Stress-Related Signature for Predicting Prognosis and Immune Features in Hepatocellular Carcinoma

Authors - Genhao Zhang
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