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TPTE Polyclonal antibody

TPTE Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12982-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 44, CT44, PTEN2, TPTE, Tumor antigen BJ HCC 5

验证数据展示

  • WB analysis of HepG2 using 12982-1-AP

    WB analysis of HepG2 using 12982-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12982-1-AP

    IHC staining of human testis using 12982-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12982-1-AP

    IHC staining of human testis using 12982-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of BxPC-3 using 12982-1-AP

    IF Staining of BxPC-3 using 12982-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 12982-1-AP (TPTE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, human testis tissue, BxPC-3 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12982-1-AP targets TPTE in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen TPTE fusion protein Ag3606 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane phosphatase with tensin homology
Synonyms Cancer/testis antigen 44, CT44, PTEN2, TPTE, Tumor antigen BJ HCC 5
Calculated Molecular Weight 62 kDa, 64 kDa
Observed Molecular Weight 62 kDa, 64 kDa
GenBank Accession NumberBC028719
Gene Symbol TPTE
Gene ID (NCBI) 7179
RRIDAB_2206336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56180
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPTE(Transmembrane phosphatase with tensin homology) is also named as CT44, BJ-HCC-5 and belongs to the non receptor class of the protein-tyrosine phosphatase family. The gene encodes a predicted polypeptide of 551 amino acids with at least 2 potential transmembrane domains and a tyrosine phosphatase motif. TPTE may be involved in the signal transduction pathways of the endocrine or spermatogenic function of the testis(PMID:10598804). It is putatively involved in linking the cell membrane to cytoskeleton. This protein is very similar in sequence to PTEN through the phosphatase and C2 domains, but lacks a PDZ binding motif and an extensively phosphorylated C-terminal tail(PMID:17240336). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TPTE antibody 12982-1-APDownload protocol
IHC protocol for TPTE antibody 12982-1-APDownload protocol
IF protocol for TPTE antibody 12982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols