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验证数据展示

  • WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TPPII Monoclonal antibody, PBS Only

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66017-1-PBS

产品说明书

CloneNo. 5H6F11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66017-1-PBS targets TPPII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5296

Product name: Recombinant human TPP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 900-1249 aa of BC039905

Sequence: LERLKDLPFIVSHRLSNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tripeptidyl peptidase II
别名 TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II
计算分子量 1249 aa, 138 kDa
观测分子量 135 kDa
GenBank蛋白编号BC039905
基因名称 TPPII
Gene ID (NCBI) 7174
RRIDAB_11044195
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29144
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

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