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TPPII Monoclonal antibody

TPPII Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5H6F11

Cat No : 66017-1-Ig

Print datasheet

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

验证数据展示

  • WB analysis of Jurkat using 66017-1-Ig

    WB analysis of Jurkat using 66017-1-Ig

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells.

  • WB analysis of HeLa using 66017-1-Ig

    WB analysis of HeLa using 66017-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66017-1-Ig

    IHC staining of human testis using 66017-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 66017-1-Ig

    IHC staining of human testis using 66017-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 66017-1-Ig

    IF Staining of HeLa using 66017-1-Ig

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of HeLa using 66017-1-Ig

    FC experiment of HeLa using 66017-1-Ig

    1X10^6 HeLa cells were stained with 0.2ug TPPII antibody (66017-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inHela cells, Jurkat
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66017-1-Ig targets TPPII in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TPPII fusion protein Ag5296 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tripeptidyl peptidase II
Synonyms TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II
Calculated Molecular Weight 1249 aa, 138 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC039905
Gene Symbol TPP2
Gene ID (NCBI) 7174
RRIDAB_11044195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

实验方案

Product Specific Protocols
WB protocol for TPPII antibody 66017-1-IgDownload protocol
IHC protocol for TPPII antibody 66017-1-IgDownload protocol
IF protocol for TPPII antibody 66017-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression.

Authors - Xinyuan Lei
View Article
humanWB

J Immunol

Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition.

Authors - Jens Dinter
View Article