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验证数据展示

  • WB analysis using 10942-1-AP (same clone as 10942-1-PBS)

    WB analysis using 10942-1-AP (same clone as 10942-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

    IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

    IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 10942-1-AP (same clone as 10942-1-PBS)

    IP experiment of mouse kidney using 10942-1-AP (same clone as 10942-1-PBS)

    IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10942-1-AP (same clone as 10942-1-PBS)

    IHC staining of human colon cancer using 10942-1-AP (same clone as 10942-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TPK1 Polyclonal antibody, PBS Only

TPK1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10942-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10942-1-PBS targets TPK1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1404

Product name: Recombinant human TPK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 11-243 aa of BC014552

Sequence: LLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thiamin pyrophosphokinase 1
别名 Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014552
基因名称 TPK1
Gene ID (NCBI) 27010
RRIDAB_2205647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3S4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

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