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TPK1 Polyclonal antibody

TPK1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10942-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human small intestine tissue, mouse testis tissue, L02 cells, mouse small intestine tissue, mouse kidney tissue, rat small intestine tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10942-1-AP targets TPK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 TPK1 fusion protein Ag1404 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thiamin pyrophosphokinase 1
别名 Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014552
基因名称 TPK1
Gene ID (NCBI) 27010
RRIDAB_2205647
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3S4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

实验方案

Product Specific Protocols
WB protocol for TPK1 antibody 10942-1-APDownload protocol
IHC protocol for TPK1 antibody 10942-1-APDownload protocol
IP protocol for TPK1 antibody 10942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.

Authors - Rohiverth Guarecuco
  • KO Validated
mouseWB

Proc Natl Acad Sci U S A

OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.

Authors - Ligong Chen
humanWB

Oncotarget

Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.

Authors - Gaganpreet S Tiwana
humanWB

Am J Pathol

Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.

Authors - Sharma Arati A
ratWB

Sci Rep

Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata.

Authors - Huimin Liu
humanWB

PLoS One

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Authors - Shuqian Liu
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