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验证数据展示

  • WB analysis using 29394-1-AP (same clone as 29394-1-PBS)

    WB analysis using 29394-1-AP (same clone as 29394-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29394-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 29394-1-AP (same clone as 29394-1-PBS)

    IHC staining of mouse eye using 29394-1-AP (same clone as 29394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29394-1-PBS in a different storage buffer formulation.

TPBG Polyclonal antibody, PBS Only

TPBG Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29394-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29394-1-PBS targets TPBG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30019

Product name: Recombinant human TPBG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 153-355 aa of NM_006670

Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trophoblast glycoprotein
别名
计算分子量 46 kDa
观测分子量72-80 kDa
GenBank蛋白编号NM_006670
基因名称 TPBG
Gene ID (NCBI) 7162
RRIDAB_2918290
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13641
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

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