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验证数据展示

  • WB analysis using 29394-1-AP

    WB analysis using 29394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 29394-1-AP

    IHC staining of mouse eye using 29394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TPBG Polyclonal antibody

TPBG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29394-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse and More (1)

应用

WB, IHC, ELISA

别名

5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, rat, mouse
文献引用反应性human
免疫原

CatNo: Ag30019

Product name: Recombinant human TPBG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 153-355 aa of NM_006670

Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trophoblast glycoprotein
别名 5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein
计算分子量 46 kDa
观测分子量72-80 kDa
GenBank蛋白编号NM_006670
基因名称 TPBG
Gene ID (NCBI) 7162
RRIDAB_2918290
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13641
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

实验方案

Product Specific Protocols
WB protocol for TPBG antibody 29394-1-APDownload protocol
IHC protocol for TPBG antibody 29394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Manag Res

MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.

Authors - Jianlong Wang
View Article
humanWB

J Cell Mol Med

In-Depth Examination of TPBG as a New Predictive Indicator for Gastric Cancer

Authors - Lianlei Yang
View Article