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验证数据展示

  • WB analysis using 86154-1-RR

    WB analysis using 86154-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86154-1-RR (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 86154-1-RR

    IHC staining of human lung cancer using 86154-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86154-1-RR (TP63 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • Affinity and Kinetic Characterization of 86154-1-RR

    Affinity and Kinetic Characterization of 86154-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86154-1-RR against Human TP63 were performed. The affinity constant is below 1 pM.

TP63 Recombinant antibody

TP63 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 86154-1-RR

产品说明书

CloneNo. 250042G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HaCaT cells, mouse skin tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86154-1-RR targets TP63 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TP63 fusion protein Ag2791 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p63
别名 p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63
计算分子量 680 aa, 77 kDa
观测分子量63-68 kDa
GenBank蛋白编号BC039815
基因名称 TP63
Gene ID (NCBI) 8626
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H3D4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).

实验方案

Product Specific Protocols
WB protocol for TP63 antibody 86154-1-RRDownload protocol
IHC protocol for TP63 antibody 86154-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols