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验证数据展示

  • WB analysis using 27988-1-AP (same clone as 27988-1-PBS)

    WB analysis using 27988-1-AP (same clone as 27988-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27988-1-AP (TOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

  • IHC staining of rat bladder using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of rat bladder using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of rat bladder using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of rat bladder using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse liver using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of mouse liver using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse liver using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of mouse liver using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse bladder using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of mouse bladder using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse bladder using 27988-1-AP (same clone as 27988-1-PBS)

    IHC staining of mouse bladder using 27988-1-AP (same clone as 27988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOX4 antibody included
  • IF Staining of HepG2 using 27988-1-AP (same clone as 27988-1-PBS)

    IF Staining of HepG2 using 27988-1-AP (same clone as 27988-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOX4 antibody (27988-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 27988-1-PBS in a different storage buffer formulation.

TOX4 Polyclonal antibody, PBS Only

TOX4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27988-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TOX 4, MIG7, LCP1, C14orf92

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TOX4 Ready-To-Use IHC Kit. TOX4 primary antibody included.

产品信息

27988-1-PBS targets TOX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27638

Product name: Recombinant human TOX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-215 aa of BC013689

Sequence: SLDSDPSLAVSDVVGHFDDLADPSSSQDGSFSAQYGVQTLDMPVGMTHGLMEQGGGLLSGGLTMDLDHSIGTQYSANPPVTIDVPMTDMTSGLMGHSQLTTIDQSELSSQLGLSLGGGTILPPAQSPEDRLSTTPSPTSSLHEDGVEDFRRQLPSQKTVVVEAGKKQKAPKKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TOX high mobility group box family member 4
别名 TOX 4, MIG7, LCP1, C14orf92
计算分子量 621 aa, 66 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC013689
基因名称 TOX4
Gene ID (NCBI) 9878
RRIDAB_3086019
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94842
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOX (Thymocyte selection-associated HMG bOX) genes represent a novel gene family and encode a novel nuclear DNA binding protein belonging to a large superfamily of HMG (high mobility group)-box family. TOX proteins consist a small subfamily of proteins, including TOX1, TOX2, TOX3, and TOX4. The TOX subfamily members are highly conserved among vertebrates. TOX4 was recently found to be one of the subunits of the PP1C, which consists of three regulatory proteins, PNUTS, TOX4, and WDR82, and one of the PP1 phosphatases, PP1α, β, andγ25. Tox4 can modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (PMID:31519808). Despite a predicted molecular mass of TOX4 protein of 66 kDa, the detection band of western blot analysis in many literatures is 100 kDa (PMID:37286708;35365735). This antibody was also detected at around 100 kda.

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