• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TOX4 Polyclonal antibody

TOX4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27988-1-AP

Print datasheet

Synonyms

C14orf92, KIAA0737, LCP1, MIG7, TOX4

验证数据展示

  • WB analysis using 27988-1-AP

    WB analysis using 27988-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27988-1-AP (TOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat bladder using 27988-1-AP

    IHC staining of rat bladder using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of rat bladder using 27988-1-AP

    IHC staining of rat bladder using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse liver using 27988-1-AP

    IHC staining of mouse liver using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse liver using 27988-1-AP

    IHC staining of mouse liver using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse bladder using 27988-1-AP

    IHC staining of mouse bladder using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included
  • IHC staining of mouse bladder using 27988-1-AP

    IHC staining of mouse bladder using 27988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOX4 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells
Positive IHC detected inrat bladder tissue, mouse bladder tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TOX4 Ready-To-Use IHC Kit. TOX4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27988-1-AP targets TOX4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen TOX4 fusion protein Ag27638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TOX high mobility group box family member 4
Synonyms C14orf92, KIAA0737, LCP1, MIG7, TOX4
Calculated Molecular Weight 621 aa, 66 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC013689
Gene Symbol TOX4
Gene ID (NCBI) 9878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94842
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOX (Thymocyte selection-associated HMG bOX) genes represent a novel gene family and encode a novel nuclear DNA binding protein belonging to a large superfamily of HMG (high mobility group)-box family. TOX proteins consist a small subfamily of proteins, including TOX1, TOX2, TOX3, and TOX4. The TOX subfamily members are highly conserved among vertebrates. TOX4 was recently found to be one of the subunits of the PP1C, which consists of three regulatory proteins, PNUTS, TOX4, and WDR82, and one of the PP1 phosphatases, PP1α, β, andγ25. Tox4 can modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (PMID:31519808). Despite a predicted molecular mass of TOX4 protein of 66 kDa, the detection band of western blot analysis in many literatures is 100 kDa (PMID:37286708;35365735). This antibody was also detected at around 100 kda.

实验方案

Product Specific Protocols
WB protocol for TOX4 antibody 27988-1-APDownload protocol
IHC protocol for TOX4 antibody 27988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols