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验证数据展示

  • WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

    WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

    IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

    IP experiment of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

    IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

    WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

    WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

    IF Staining of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TOP2B Polyclonal antibody, PBS Only

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20549-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20549-1-PBS targets TOP2B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 topoisomerase (DNA) II beta 180kDa
别名
计算分子量 183 kDa
观测分子量 183 kDa
GenBank蛋白编号NM_001068
基因名称 TOP2B
Gene ID (NCBI) 7155
RRIDAB_10700004
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02880
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

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