验证数据展示

WB analysis of HeLa using 20549-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 20549-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens).
IP experiment of HepG2 using 20549-1-AP
IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug.
WB analysis of HepG2 using 20549-1-AP
WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.
IF Staining of HeLa using 20549-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

TOP2B Polyclonal antibody

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20549-1-AP

产品说明书

引用文献(14)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

DNA topoisomerase 2 beta, TOP2B, top2beta, TOPIIB

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (14)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, HepG2 cells, K-562 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 2 publications below
FC	See 1 publications below
ChIP	See 1 publications below

产品信息

20549-1-AP targets TOP2B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, ChIP
经测试反应性	human
文献引用反应性	human, mouse
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	topoisomerase (DNA) II beta 180kDa
别名	DNA topoisomerase 2 beta, TOP2B, top2beta, TOPIIB
计算分子量	183 kDa
观测分子量	183 kDa
GenBank蛋白编号	NM_001068
基因名称	TOP2B
Gene ID (NCBI)	7155
RRID	AB_10700004
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q02880
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

实验方案

Product Specific Protocols
IF protocol for TOP2B antibody 20549-1-AP	Download protocol
IHC protocol for TOP2B antibody 20549-1-AP	Download protocol
IP protocol for TOP2B antibody 20549-1-AP	Download protocol
WB protocol for TOP2B antibody 20549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Science Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. Authors - Anand Minajigi View Article
human	WB,IP	Mol Cell MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome". Authors - Subhendu K Das View Article
human	WB	J Med Chem Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma. Authors - Zongyuan Zhou View Article
human	WB	RNA DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells Authors - Maya David View Article
mouse	WB	Sci Rep Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy. Authors - Fei Xu KD Validated View Article
human		Sci Rep Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin. Authors - Lin-Yang Sun View Article

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验证数据展示

WB analysis of HeLa using 20549-1-AP

IHC staining of human cervical cancer using 20549-1-AP

IP experiment of HepG2 using 20549-1-AP

WB analysis of HepG2 using 20549-1-AP

IF Staining of HeLa using 20549-1-AP

TOP2B Polyclonal antibody

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells

Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.

Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.