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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85773-4-RR (same clone as 85773-4-PBS)

    WB analysis using 85773-4-RR (same clone as 85773-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85773-4-RR (TOMM40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85773-4-RR (same clone as 85773-4-PBS)

    IF Staining of HepG2 using 85773-4-RR (same clone as 85773-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM40 antibody (85773-4-RR, Clone: 250010E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85773-4-RR

    Affinity and Kinetic Characterization of 85773-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85773-4-RR against Human TOMM40 were performed. The affinity constant is 1.59 nM.

TOMM40 Recombinant antibody, PBS Only

TOMM40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85773-4-PBS

产品说明书

CloneNo. 250010E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

TOM40, C19orf1, D19S1177E, p38.5, PER EC1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85773-4-PBS targets TOMM40 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TOMM40 fusion protein Ag13065 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 40 homolog (yeast)
别名 TOM40, C19orf1, D19S1177E, p38.5, PER EC1
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC017224
基因名称 TOMM40
Gene ID (NCBI) 10452
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO96008
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.