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验证数据展示

  • IHC staining of mouse liver using 85773-4-RR

    IHC staining of mouse liver using 85773-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85773-4-RR (TOMM40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 85773-4-RR

    IHC staining of mouse liver using 85773-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85773-4-RR (TOMM40 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 85773-4-RR

    WB analysis using 85773-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85773-4-RR (TOMM40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85773-4-RR

    IF Staining of HepG2 using 85773-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM40 antibody (85773-4-RR, Clone: 250010E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85773-4-RR

    Affinity and Kinetic Characterization of 85773-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85773-4-RR against Human TOMM40 were performed. The affinity constant is 1.59 nM.

TOMM40 Recombinant monoclonal antibody

TOMM40 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85773-4-RR

产品说明书

CloneNo. 250010E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

TOM40, C19orf1, D19S1177E, p38.5, PER EC1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85773-4-RR targets TOMM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13065

Product name: Recombinant human TOMM40 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-361 aa of BC017224

Sequence: MGNVLAASSPPAGPPPPPAPALVGLPPPPPSPPGFTLPPLGGSLGAGTSTSRSSERTPGAATASASGAAEDGACGCLPNPGTFEECHRKCKELFPIQMEGVKLTVNKGLSNHFQVNHTVALSTIGESNYHFGVTYVGTKQLSPTEAFPVLVGDMDNSGSLNAQVIHQLGPGLRSKMAIQTQQSKFVNWQVDGEYRGSDFTAAVTLGNPDVLVGSGILVAHYLQSITPCLALGGELVYHRRPGEEGTVMSLAGKYTLNNWLATVTLGQAGMHATYYHKASDQLQVGVEFEASTRMQDTSVSFGYQLDLPKANLLFKGSVDSNWIVGATLEKKLPPLPLTLALGAFLNHRKNKFQCGFGLTIG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 40 homolog (yeast)
别名 TOM40, C19orf1, D19S1177E, p38.5, PER EC1
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC017224
基因名称 TOMM40
Gene ID (NCBI) 10452
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO96008
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

实验方案

Product Specific Protocols
IF protocol for TOMM40 antibody 85773-4-RRDownload protocol
WB protocol for TOMM40 antibody 85773-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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