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验证数据展示

  • WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

    WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 32179-1-AP (same clone as 32179-1-PBS)

    WB analysis of mouse brain using 32179-1-AP (same clone as 32179-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

  • WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

    WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 32179-1-AP (same clone as 32179-1-PBS)

    IF Staining of HeLa using 32179-1-AP (same clone as 32179-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TOM1L2 antibody (32179-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

    IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

    IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

TOM1L2 Polyclonal antibody, PBS Only

TOM1L2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32179-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Target Of Myb1 Like 2 Membrane Trafficking Protein, Target of Myb-like protein 2, TOM1-like protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32179-1-PBS targets TOM1L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37612

Product name: Recombinant human TOM1L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-457 aa of NM_001082968.1

Sequence: LRYERFERYRSGRSVQNASNGVLNEVTEDNLIDLGPGSPAVVSPMVGNTAPPSSLSSQLAGLDLGTESVSGTLSSLQQCNPRDGFDMFAQTRGNSLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLRTDLKGDDLEEGVTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 target of myb1-like 2 (chicken)
别名 Target Of Myb1 Like 2 Membrane Trafficking Protein, Target of Myb-like protein 2, TOM1-like protein 2
计算分子量56 kDa，507aa
观测分子量60 kDa
GenBank蛋白编号NM_001082968.1
基因名称 TOM1L2
Gene ID (NCBI) 146691
RRIDAB_3670215
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZVM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).

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