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验证数据展示

  • WB analysis using 32179-1-AP

    WB analysis using 32179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 32179-1-AP

    WB analysis of mouse brain using 32179-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 32179-1-AP

    WB analysis using 32179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 32179-1-AP

    IF Staining of HeLa using 32179-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TOM1L2 antibody (32179-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

TOM1L2 Polyclonal antibody

TOM1L2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32179-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

TOM1-like protein 2, Target of Myb-like protein 2, Target Of Myb1 Like 2 Membrane Trafficking Protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A-253 cells, BxPC-3 cells, HaCaT cells, LNCaP cells, U-251 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32179-1-AP targets TOM1L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TOM1L2 fusion protein Ag37612 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 target of myb1-like 2 (chicken)
别名 TOM1-like protein 2, Target of Myb-like protein 2, Target Of Myb1 Like 2 Membrane Trafficking Protein
计算分子量56 kDa，507aa
观测分子量60 kDa
GenBank蛋白编号NM_001082968.1
基因名称 TOM1L2
Gene ID (NCBI) 146691
RRIDAB_3670215
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZVM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).

实验方案

Product Specific Protocols
WB protocol for TOM1L2 antibody 32179-1-APDownload protocol
IF protocol for TOM1L2 antibody 32179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols