验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A-253 cells, BxPC-3 cells, HaCaT cells, LNCaP cells, U-251 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32179-1-AP targets TOM1L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37612 Product name: Recombinant human TOM1L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-457 aa of NM_001082968.1 Sequence: LRYERFERYRSGRSVQNASNGVLNEVTEDNLIDLGPGSPAVVSPMVGNTAPPSSLSSQLAGLDLGTESVSGTLSSLQQCNPRDGFDMFAQTRGNSLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLRTDLKGDDLEEGVTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | target of myb1-like 2 (chicken) |
| 别名 | Target Of Myb1 Like 2 Membrane Trafficking Protein, Target of Myb-like protein 2, TOM1-like protein 2 |
| 计算分子量 | 56 kDa，507aa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_001082968.1 |
| 基因名称 | TOM1L2 |
| Gene ID (NCBI) | 146691 |
| RRID | AB_3670215 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6ZVM7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TOM1L2 antibody 32179-1-AP | Download protocol |
| IHC protocol for TOM1L2 antibody 32179-1-AP | Download protocol |
| WB protocol for TOM1L2 antibody 32179-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
