验证数据展示
经过测试的应用
| Positive WB detected in | BT-474 cells, SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
51017-1-AP targets TOM1L1 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0502 Product name: Recombinant human TOM1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-1/1 of BC029396 Sequence: 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | target of myb1 (chicken)-like 1 |
| 别名 | Src-activating and signaling molecule protein, Target of Myb-like protein 1, TOM1 L1, TOM1 like protein 1, TOM1L1 protein |
| 计算分子量 | 476 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC029396 |
| 基因名称 | TOM1L1 |
| Gene ID (NCBI) | 10040 |
| RRID | AB_2207419 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Target of Myb-like protein 1 (TOM1L1), also known as Srcasm, belongs to the Tom family. TOM1L1 encodes an adaptor protein characterized by an N-terminal VHS domain and a central GAT domain, which facilitates interactions with clathrin and components of the endosomal sorting machinery. Functionally, TOM1L1 serves as a substrate for Src family kinases, playing a regulatory role in growth factor signaling and membrane trafficking. It is involved in endocytosis, signal transduction, and the negative regulation of mitotic cell division. (PMID: 17977829, PMID: 26899482, PMID: 35238864, PMID: 39548458)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOM1L1 antibody 51017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
