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验证数据展示

  • WB analysis of HeLa using 14915-1-AP (same clone as 14915-1-PBS)

    WB analysis of HeLa using 14915-1-AP (same clone as 14915-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14915-1-AP (TOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14915-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 14915-1-AP (same clone as 14915-1-PBS)

    IHC staining of human stomach cancer using 14915-1-AP (same clone as 14915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14915-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 14915-1-AP (same clone as 14915-1-PBS)

    IHC staining of human stomach cancer using 14915-1-AP (same clone as 14915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14915-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14915-1-AP (same clone as 14915-1-PBS)

    IP experiment of HeLa using 14915-1-AP (same clone as 14915-1-PBS)

    IP result of anti-TOB1 (IP:14915-1-AP, 4ug; Detection:14915-1-AP 1:1000) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 14915-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TOB1 Polyclonal antibody, PBS Only

TOB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14915-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14915-1-PBS targets TOB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6714

Product name: Recombinant human TOB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-345 aa of BC031406

Sequence: MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPEKPYKGSGFRCIHIGEKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDNNENGCELDKEIKNSFNPEAQVFMPISDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSNKVARTSPINLGLNVNDLLKQKAISSSMHSLYGLGLGSQQQPQQQQQPAQPPPPPPPPQQQQQQKTSALSPNAKEFIFPNMQGQGSSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQYSNQQFQPVMAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transducer of ERBB2, 1
别名
计算分子量 38 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC031406
基因名称 TOB1
Gene ID (NCBI) 10140
RRIDAB_10896496
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50616
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

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