TOB1 Polyclonal antibody
TOB1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14915-1-AP targets TOB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | TOB1 fusion protein Ag6714 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transducer of ERBB2, 1 |
| Synonyms | APRO6, PIG49, Protein Tob1, TOB, TOB1, Transducer of erbB 2 1, transducer of ERBB2, 1, TROB, TROB1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38-45 kDa |
| GenBank Accession Number | BC031406 |
| Gene Symbol | TOB1 |
| Gene ID (NCBI) | 10140 |
| RRID | AB_10896496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50616 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOB1 antibody 14915-1-AP | Download protocol |
| IHC protocol for TOB1 antibody 14915-1-AP | Download protocol |
| IF protocol for TOB1 antibody 14915-1-AP | Download protocol |
| IP protocol for TOB1 antibody 14915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network. | ||
RNA Biol NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation. | ||
Am J Transl Res Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression. | ||
Commun Biol TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression
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