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TOAG1 Polyclonal antibody

TOAG1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21564-1-AP

Print datasheet

Synonyms

C3orf23, TCAIM, T-cell activation inhibitor, TOAG1

验证数据展示

  • IHC staining of human liver using 21564-1-AP

    IHC staining of human liver using 21564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 21564-1-AP

    WB analysis of HEK-293 using 21564-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21564-1-AP (TOAG1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below
IHCSee 1 publications below

产品信息

The immunogen of 21564-1-AP is TOAG1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen TOAG1 fusion protein Ag16067 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 3 open reading frame 23
Synonyms C3orf23, TCAIM, T-cell activation inhibitor, TOAG1
Calculated Molecular Weight 496 aa, 58 kDa
Observed Molecular Weight 35-41 kDa
GenBank Accession NumberBC101060
Gene Symbol C3orf23
Gene ID (NCBI) 285343
RRIDAB_2878885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3R3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOAG1, also named as TCAIM and C3orf23, is a mitochondrial protein which regulates T-cell apoptosis. It is a specific and reproducible marker for quiescence. TOAG1 has two isoforms with MW 41 kDa and 58 kDa.

实验方案

Product Specific Protocols
WB protocol for TOAG1 antibody 21564-1-APDownload protocol
IHC protocol for TOAG1 antibody 21564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

FEBS J

A novel role of TCAIM: suppressing renal carcinoma growth and enhancing its sensitivity to sunitinib

Authors - Zhuo Ye
  • KD Validated
View Article