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验证数据展示

  • WB analysis of mouse lung using 25353-1-AP

    WB analysis of mouse lung using 25353-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25353-1-AP

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CELF3 antibody included
  • IHC staining of human testis using 25353-1-AP

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CELF3 antibody included

TNRC4 Polyclonal antibody

TNRC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25353-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CELF3 Ready-To-Use IHC Kit. CELF3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25353-1-AP targets TNRC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18601

Product name: Recombinant human TNRC4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 180-352 aa of BC052491

Sequence: GLRRMQQVATQLGMFSPITLQFGAYSAYTQALMQQQAALVAAHSAYLSPMATMAAVQMQHMAAINANGLIATPITPSSGTSTPPAIAATPVSAIPAALGVNGYSQVPTQPTGQPAPDALYPNGVHPYPAQSPAAPVDPLQQAYAGMQHYTAYPAAYSLVAPAFPQPPALVAQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trinucleotide repeat containing 4
别名 Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4
计算分子量 464 aa, 51 kDa
观测分子量 55 kDa
GenBank蛋白编号BC052491
基因名称 TNRC4
Gene ID (NCBI) 11189
RRIDAB_2880041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SZQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNRC4, also named as BRUNOL1, CAGH4, ERDA4, or TNRC4, is a 465 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the CELF/BRUNOL family. TNRC4 localizes in the nucleus and is expressed in brain. TNRC4 as a RNA-binding protein is involved in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TNRC4 antibody 25353-1-APDownload protocol
IHC protocol for TNRC4 antibody 25353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols