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验证数据展示

  • IF Staining of U2OS using CL488-13504

    IF Staining of U2OS using CL488-13504

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 TNNC1 antibody (CL488-13504) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TNNC1 Polyclonal antibody

TNNC1 Polyclonal Antibody for IF/ICC
Cat No. CL488-13504

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CMD1Z, TN C, TNC, TNNC, TNNC1, troponin C type 1 (slow)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 13504-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13504 targets TNNC1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4339

Product name: Recombinant human TNNC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-161 aa of BC030244

Sequence: MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin C type 1 (slow)
别名 CMD1Z, TN C, TNC, TNNC, TNNC1, troponin C type 1 (slow)
计算分子量 161 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC030244
基因名称 TNNC1
Gene ID (NCBI) 7134
RRIDAB_3672568
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63316
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TNNC1 antibody CL488-13504Download protocol
Standard Protocols
Click here to view our Standard Protocols