验证数据展示
经过测试的应用
| Positive WB detected in | SW480 cells |
| Positive IP detected in | HeLa cells |
| Positive FC (Intra) detected in | SW480 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
24999-1-AP targets TNFRSF6B in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19781 Product name: Recombinant human TNFRSF6B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC017065 Sequence: MRALEGPGLSLLCLVLALPALLPVPAVRGVAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor receptor superfamily, member 6b, decoy |
| 别名 | DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, M68, TR6 |
| 计算分子量 | 300 aa, 33 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC017065 |
| 基因名称 | TNFRSF6B |
| Gene ID (NCBI) | 8771 |
| RRID | AB_2879838 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95407 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TNFRSF6B antibody 24999-1-AP | Download protocol |
| WB protocol for TNFRSF6B antibody 24999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
