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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL647-98056

    FC experiment of human PBMCs using CL647-98056

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human TNF beta Rabbit RecAb (CL647-98056, Clone:240393C3). Cells were co-stained with FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647 Anti-Human TNF beta Rabbit Recombinant Antibody

TNF beta Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL647-98056

产品说明书

CloneNo. 240393C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

LTA, 240393C3, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inAnti-CD3/CD28 treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 98056-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98056 targets TNF beta in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg0836

Product name: Recombinant Human TNF-beta protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 35-205 aa of BC034729

Sequence: LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphotoxin alpha (TNF superfamily, member 1)
别名 LTA, 240393C3, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha
计算分子量 205 aa, 22 kDa
GenBank蛋白编号BC034729
基因名称 TNF beta
Gene ID (NCBI) 4049
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01374
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TNF beta antibody CL647-98056Download protocol
Standard Protocols
Click here to view our Standard Protocols