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验证数据展示

  • WB analysis using 67710-1-Ig (same clone as 67710-1-PBS)

    WB analysis using 67710-1-Ig (same clone as 67710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included
  • IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included
  • IF Staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)

    IF Staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TNC/Tenascin-C antibody (67710-1-Ig, Clone: 1F7G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

TNC/Tenascin-C Monoclonal antibody, PBS Only

TNC/Tenascin-C Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67710-1-PBS

产品说明书

CloneNo. 1F7G8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TNC/Tenascin-C Ready-To-Use IHC Kit. TNC/Tenascin-C primary antibody included.

产品信息

67710-1-PBS targets TNC/Tenascin-C in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag21412

Product name: Recombinant human TNC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1852-2201 aa of BC151843

Sequence: YALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tenascin C
别名 Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC
计算分子量 2201 aa, 241 kDa
观测分子量260 kDa
GenBank蛋白编号BC151843
基因名称 Tenascin C
Gene ID (NCBI) 3371
RRIDAB_2882900
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP24821
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

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